Journal: Journal of Human Genetics

Article Title: Compound heterozygous variants in MYH11 underlie autosomal recessive megacystis-microcolon-intestinal hypoperistalsis syndrome in a Chinese family

doi: 10.1038/s10038-019-0651-z

Figure Lengend Snippet: Compound heterozygous variants in MYH11 in a family with MMIHS. a Prenatal ultrasonography image at 13 weeks of gestation for the index fetus demonstrated a distended bladder (2.56 cm × 2.32 cm). b Prenatal ultrasonography image at 17 weeks shows a progressive distention of the bladder (9.5 cm × 7.16 cm) in the index fetus. c Sanger sequencing validates the exome sequencing variant of c.2051 G > A (p.R684H) in MYH11 (NM_001040114). d Sanger sequencing validates the exome sequencing variant of c.3540_3541delinsTT (p.(E1180D, Q1181Ter)) in MYH11 (NM_001040114). e Protein expression of MYH11 in the control (Ctrl) and proband umbilical cord tissues. Arrows point to the band location for protein MYH11

Article Snippet: Anti-MYH11 antibody (1:50, Monoclonal rabbit IgG, BM5659, Boster Biological Technology, China) was used as a primary antibody.

Techniques: Sequencing, Variant Assay, Expressing, Control

Journal: Journal of Human Genetics

Article Title: Compound heterozygous variants in MYH11 underlie autosomal recessive megacystis-microcolon-intestinal hypoperistalsis syndrome in a Chinese family

doi: 10.1038/s10038-019-0651-z

Figure Lengend Snippet: Summary of clinical and molecular findings of four genes involved in autosomal recessive MMIHS

Article Snippet: Anti-MYH11 antibody (1:50, Monoclonal rabbit IgG, BM5659, Boster Biological Technology, China) was used as a primary antibody.

Techniques: Sequencing

Journal: The Prostate

Article Title: The identification of a novel antibody for CD133 using human antibody phage display

doi: 10.1002/pros.23656

Figure Lengend Snippet: Case study of a patient with aggressive prostate cancer. A, CT scan of diffuse liver metastases that appeared in the presence of declining PSA after the patient failed treatment with Zytiga (abiraterone). B, Strong staining for CD133 was detected in the liver metastases using HA10. C, No staining for the AR was observed in the liver metastases using an antibody specific to the N-terminal domain of the AR. This antibody would detect both full-length AR and AR splice variants. D, Staining was observed for the neuroendocrine differentiation marker chromogranin A in the liver metastases. E, An original TURP specimen from the patient depicting poorly differentiated Gleason 4 + 5 cancer stained for CD133. Note the complete absence of CD133. F, Liver biopsy from the patient after failing treatment with carboplatin and taxotere documenting moderate staining for CD133. Scale bars, (B-D) 200 μm and (E-F) 60 μm. [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: The following primary antibodies and concentrations used were: CD133 HA10 antibody, rabbit monoclonal (1:100); androgen receptor SP107 (Sigma), rabbit monoclonal (1:200); chromogranin A PB9097 (Boster Biological Technology) rabbit polyclonal (1:1000); CD133/1 (AC133) (Miltenyi) mouse monoclonal (1:25).

Techniques: Computed Tomography, Staining, Marker